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KMID : 1007520230320060803
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Food Science and Biotechnology
2023 Volume.32 No. 6 p.803 ~ p.811
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Duplex droplet digital PCR (ddPCR) for simultaneous quantification of bovine and porcine gelatin in capsules
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Pornpimol Mahamad
Winai Dahlan
Saveeyah Kahong
Sukanya So-audon
Wila Munaowaroh
Vanida Nopponpunth
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Abstract
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Detection of bovine and porcine in gelatin-based products is important as species fraud and product mislabeling may have a detrimental impact on customers who have health, ethical, and religious concerns about animal products. The duplex droplet digital PCR (ddPCR) assay using double-quenched probes has been developed for quantification and detection of porcine and bovine DNA in gelatin capsules. A DNA mixture derived from gelatin was found to have a limit of detection as low as 0.001 ng/μl for porcine samples and 0.01 ng/μl for bovine samples. DNA from 12 other distinct species was tested with the bovine and porcine probes, showing high specificity for this method. The test was validated using fifty-five commercial supplement and pharmaceutical capsules, of which 17 were positive for bovine and/or porcine DNA. This study shows that the duplex ddPCR is reliable for routine analysis in the identification of bovine and porcine origins for gelatin capsules.
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KEYWORD
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ddPCR, Porcine, Bovine, Gelatin, Capsule
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